For analysis of CTE pathologic severity, AT8 (ptau) immunostained slides from the DLFC were scanned and digitized at 20x magnification using the Aperio ScanScope (Leica) as previously described [ 42 ]. Identification, selection, and analysis of the regions of interest were performed as previously described [ 6 ]. Briefly, the depth of the cortical sulcus, which was defined as the bottom third of two connecting gyri, was selected and circled in ImageScope (Lecia). Only gray matter was highlighted. A modified version of the Aperio positive pixel count algorithm (Version 9) was used to determine the total area of AT8 positive staining. Quantifications were standardized to the area measured and presented as density per analyzed area as previously described [ 6 ].
On June 10, 2005, Iwo J. Bohr's new hypothesis was released from peer review and published in Lipids in Health and Disease , hypothesizing that cholesterol is a protective factor in Alzheimer's disease. 28 As background, Borh cites a hypercholesterolemic diet as protective of cognitive function in rats and a high cholesterol level's association in humans with lower mortality and a better outcome following a first stroke. He also points out that Alzheimer's patients have lower levels of cholesterol in serum, brain membranes, and in lipid rafts, and that Alzheimer's has been related to the downregulation of a gene involved in cholesterol synthesis.
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